Argonne National Laboratory


Users Highlights

Department of Energy Office of Science
GM/CA @ APS Sponsors:
National Institute of General Medical Sciences (NIGMS) and National Cancer Institute (NCI) of the National Institutes of Health (NIH)

Structure of enzyme domain involved in synthesis of natural products

Janet Smith group (University of Michigan) and collaborators


Janet Smith's group from the University of Michigan used the beamline automounter to screen ~48 crystals in ~4 hrs to solve the structure of the ECH2 domain of CurF from Lyngbya majuscula. CurF ECH2 is a decarboxylase involved in the introduction of a cyclopropyl ring in the biosynthesis of the natural product curacin A, which is a potent cytotoxic agent. The structure was solved with a 3-wavelength MAD data set using selenomethionine-labeled protein on beamline 23ID-B. The structure demonstrated that CurF ECH2 has a crotonase fold, and substrate modeling into the active site facilitated testing of active site residues. This new structure has provided insights into β-branching in polyketide synthesis as described in a manuscript submitted for publication.

Figure: View of CurF ECH2 monomer demonstrating crotonase superfamily fold and active site chamber highlighted with red asterisk.


Geders, TW, Gu, L, Mowers, JC, Liu, H, Gerwick, WH, Hakansson, K, Sherman, DH, Smith, JL. "Crystal Structure of the ECH2 Catalytic Domain of CurF from Lyngbya majuscula: Insights into a Decarboxylase Involved in Polyketide Chain β-Branching", J. Biol. Chem. 282 (49), 35954-35963 (2007). DOI: 10.1074/jbc.M703921200.

GM/CA @ APS is an Office of Science User Facility operated for the U.S. Department of Energy Office of Science by Argonne National Laboratory

UChicago Argonne LLC | Privacy & Security Notice | Contact Us | A-Z Index | Search